Factor VIII Related Antigen (vWF, von Willebrand Factor)

Size

£1,107.00

SKU:: F0016-13C
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: von Willebrand Factor (Factor VIII related antigen) from human plasma.
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Human von Willebrand factor (factor VIII R:Ag) is a 270kD multimeric plasma gylcoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:200-1:400 using indirect Immunoflourescence. Enzymatically pre-digested with 0.1% pronase, formalin fixed. Specifically stains endothelial cells. Immunohistochemistry (Paraffin): 1:1000 using indirect immunoperoxidase. Enzymatically pre-digested with 0.1% pronase, formalin fixed and developed with Biotin/Avidin. Specifically stains blood vessels. Optimal dilutions to be determined by the researcher. Positive Control: Formalin-fixed, paraffin sections of human tongue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice