F0016-07
Factor VIII
Size
£793.00
- SKU:
- F0016-07
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Affinity purified human Factor VIII
- Clone:
- 7E127 (RFFVIIIC/8)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerizes and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause hemophilia A, a disorder characterized by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Clone 7E127 (RFFVIIIC/8) is a very potent coagulation inhibitor. Applications: Suitable for use in ELISA, Western Blot and RIA. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:15,000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-04D: IgG, H&L (AP) Pab Gt xMo I1904-06F: IgG, H&L (FITC) Pab Gt xMo I1904-06C: IgG, H&L (HRP) Pab Gt xMo I1903-07E: IgG, (HRP) Pab Rb xMo I1903-13A: IgG, (PE) Pab Gt xMo Hybridoma: P3-NS/a-AG4-1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice