F0015

Factor VII (F.VII, Stable Factor, Proconvertin)

Size

£499.00

SKU:: F0015
Purity:: Purified by gel filtration and ion exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human Factor VII
Clone:: 8.H.3
Format:: Supplied as a liquid in ddH2O, 50% glycerol
Extra Details:: Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor. After cleavage of a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39 to 190) and the heavy chain (residues 191 to 444). Applications: Suitable for RIA, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Coating: 5-10ug/ml; Secondary: 100-1000ug/ml Western Blot (Reduced and oxidized forms): 5ug/ml Optimal dilutions to be determined by the researcher. Extinction Coefficient: E1%, 1cm, 280nm = 14.0 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.