F0015-02
Factor VII (F.VII, Stable Factor, Proconvertin)
Size
£843.00
- SKU:
- F0015-02
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala39-Pro444 from human Factor VII expressed in NSO cells. Species Sequence Homology: canine - 76%, mouse - 71%, rat - 70%, chicken - 57%
- Clone:
- 7H85
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml detected recombinant human Factor VII. Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Factor VII. Immunocytochemistry: 8-25ug/ml using immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice