E9108-48B
eIF-2alpha (EIF2S1, EIF2, EIF-2, EIF2A, eIF-2A, EIF-2A, EIF-2alpha, eIF-2-alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa)
Size
£581.00
- SKU:
- E9108-48B
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the C-terminal sequence of eIF-2alpha.
- Format:
- Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B (1,2). Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2- GDP-eIF2B complex, inhibiting the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2alpha at Ser51 (5,6). Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice