E9100-01B
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Size
£800.00
- SKU:
- E9100-01B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated, synthetic peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG). Species sequence homology: rat
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
- Extra Details:
- ERM (Ezrin-Radixin-Moesin) proteins function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane. One function of ezrin is in determining survival of epithelia cells by activating the P1-3-kinase/Akt pathway. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detected Ezrin in RIPA lysates from A431 cells and a mourine brain lysate. Immunohistochemistry: 10ug/ul detects Ezrin in paraffin embedded human skin tissue sections. Optimal dilutions to be determined by the researcher. Positive Control Included: A0001-50: A431 Cell Lysate, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice