E9005-07B1

Exodus 2 (Exodus-2, 6Ckine, Beta Chemokine Exodus-2, Chemokine (C-C Motif) Ligand 21, CCL21, CKb9, ECL, Secondary Lymphoid Tissue Chemokine, SLC, Small Inducible Cytokine A21, SCYA21, TCA4) (Biotin)

Size

£629.00

SKU:: E9005-07B1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant human Exodus-2, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile ddH2O, 0.1% BSA.
Extra Details:: Exodus-2 is expressed in the HEV and T cell rich areas of lymph nodes and Peyer’s patches, T cell areas of the spleen and by lymphatic endothelium of many organs, acting as a potent chemoattractant for thymocytes and T lymphocytes. Exodus-2 possesses characteristics of a homing molecule for lymphocytes to secondary lymphoid organs and has been shown to inhibit haematopoiesis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: using sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25 -1ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with E9005-06 as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human Exodus-2. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Exodus-2 is 1.5 - 3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice