E8949-38T
Eukaryotic Translation Initiation Factor 4B (eIF4B)
Size
£825.00
- SKU:
- E8949-38T
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant protein corresponding to Met1-Arg200 from human eIF-4B.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- eIF-4B (eukaryotic translation initiation factor 4B), an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF-2, GTP, and Met-tRNA stabilized by eIF-3. An arginine rich motif (ARM) in the carboxy-terminus of eIF-4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF-4B in conjunction with eIF-4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF-4E. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml with A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice