Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

Size

£863.00

SKU:: E8949-01C
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant human eIF-2a (rheIF-2a, aa1-315)
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: EIF-2a (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the eIF-2 protein, an important regulator of translation initiation. Phosphorylation of eIF-2a on Ser 51 increases the affinity of eIF-2a for eIF-2B, a guanine nucleotide exchange factor needed for the recycling of eIF-2-GDP to eIF-2-GTP. Reduction of eIF-2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF-2a kinases. Applications: Western Blot: An antibody concentration of 1ug/mL is recommended. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.