E8949-01
Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)
Size
£601.00
- SKU:
- E8949-01
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide 13aa of Rat eIF-2alpha phosphorylated (KLH).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis (1). Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses (2). EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52 kD) (3). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Western Blot (ECL): 1ug/ml Optimal dilutions to be determined by researcher. Positive Controls: HeLa Cell Lysate: Use 20ug per lane. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice