ETFDH (Electron Transfer Flavoprotein-ubiquinone Oxidoreductase, Mitochondrial, ETF-ubiquinone Oxidoreductase, ETF-QO, Electron-transferring-flavoprotein Dehydrogenase, ETF Dehydrogenase)

Size

£642.00

SKU:: E8445-50A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-EHDQPAHLTLRD, from ETFDH, at the internal region of the protein (NP_004444.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67kD precursor which is targeted to mitochondria and processed in a single step to a 64kD mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 1-3ug/ml, observed in human, mouse and rat heart lysates on ~70kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.