Estrogen Receptor, beta (ERb) (MaxLight 650)

Size

£935.00

SKU:: E3565-17-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 19 amino acid synthetic peptide derived from the C-terminus of the human Estrogen Receptor beta protein.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Estrogen receptor (ER) is a member of the steroid-receptor family. Unlike protein growth factors that bind to receptors on the cell surface and activate signal-transduction cascades to influence gene expression, the steroid hormones bind to intracellular receptors, which then bind to DNA and regulate gene expression directly. ER-b has a molecular mass of approximately 53kD and is composed of six regions, A to F, on the basis of different amino acid sequence homology (2). The A/B region contains a transcription activation function. Region C encodes the DNA binding domain. Region D is involved in binding to hsp90, as well as containing nuclear localization signals and plays a part in stabilizing DNA binding by the DNA binding domain. Region E contains the hormone/ligand binding domain as well as hormone-inducible transcription activation function. Region F appears to play a role in modulating transcriptional activation by ER-a. ER-b was discovered in 1996 in rat prostate, as well as in secretory epithelial cells of the prostate and granulose cells of the ovary (1). Later, human ER-b was discovered and characterized and found to be expressed in human thymus, spleen, ovary and testis (3). Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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