Estrogen Receptor beta (ERb)

Size

£735.00

SKU:: E3565-16M
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa467-485, C-CSTEDSKNKEGSQNLQSQ of C-terminal mouse Estrogen Receptor beta (isoform beta6).
Format:: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Extra Details:: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunofluorescence: 1:50-1:200 Immunohistochemistry (Paraffin): 1-2ug/ml Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice