E3565-11Z1
Estrogen Receptor alpha (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor 1)
Size
£863.00
- SKU:
- E3565-11Z1
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Thr2-Gln116 from human ERa/NR3A1 expressed in E. coli. Species Sequence Homology: mouse
- Clone:
- 11n68
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- ERa (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ERa is normally quiescent and bound to heat-shock proteins and immunophilins. Following b-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ERb, and binds to DNA with multiple coactivators. Human ERa is 595aa in length. It contains a DNA binding region (aa185-250), three NLSs (aa256-260; 266-271; 299-303), a steroid-binding site (aa351-543), a dimerization motif (aa497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at aa537, aa167 and aa118. Multiple splice forms exist. There is an 80kD isoform that shows a substitution (duplication) of aa412-517 for aa411, a second isoform with a deletion of aa255-366, a third isoform with a deletion of aa152-412, and a fourth isoform that shows a Thr substitution for aa152-595. Human ERa is only 46aa identical to human ERb. Over aa1-116, human ERa shares 85aa identity with mouse ERa. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml using Immersion fixed paraffin-embedded sections of human breast after HEIR. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice