E3565-09T

Estrogen Receptor, alpha (ERa)

Size

£825.00

SKU:: E3565-09T
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa247-261 (EVGMMKGGIRKDRRG) of the DNA binding domain of ER-alpha (estrogen receptor-alpha). Species Sequence Homology: porcine, sheep, chicken and frog
Clone:: 33
Format:: Supplied as a liquid in DPBS, 1mg/ml BSA, 0.05% sodium azide.
Extra Details:: Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies. Applications: Suitable for use in ImmunocytochemistryImmunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen/paraffin): 1:10-1:500 Immunocytochemistry (IF): 10ug/ml Western Blot: 1ug/ml. 50ug bovine liver was analyzed. Blocking was in 5% milk in PBST for 1 hour. E3565-09T diluted to 1ug/ml was incubated with blocking buffer for 1 hour RT. Three x 8 minute washes were performed, then secondary antibody added, repeat washes, then ECL reagents used. Film exposure determined positive signal. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice