E3508-35
ESGP (ES Cell and Germ Cell-specific Protein)
Size
£965.00
- SKU:
- E3508-35
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Leu24-Lys84 from mouse ESGP, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul PBS.
- Extra Details:
- ESGP (ES cell and germ cell-specific protein) is a presumably secreted polypeptide that belongs to no known protein family. It is expressed specifically in pluripotential cell types, and does not appear to be involved in differentiation. Mouse ESGP precursor is 84 amino acids (aa) in length. It contains a 25 aa signal sequence with a 59 aa mature segment. There are no N-linked glycosylation sites. One potential 108 aa variant shows an alternate start site 24 aa upstream of the standard start site. Applications: Suitable for use in Direct ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml detects ESGP in lysates of D3 mouse embryonic stem cell line Immunocytochemistry: 5-15ug/ml in immersion fixed D3 mouse embryonic stem cell Immunohistochemistry: 5-15ug/ml in perfusion fixed frozen sections of mouse testis Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice