ESDN (Endothelial and Smooth Muscle Cell-derived Neuropilin-like Molecule, Endothelial and Smooth Muscle Cell-derived Neuropilin-like Protein, 1700055P21Rik, Coagulation Factor V/VIII-homology Domains Protein 1, CLCP1, Discoidin CUB and LCCL Domain Containing 2, DCBLD2)

Size

£908.00

SKU:: E3506-02A
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Gln67-Ala528 from human ESDN expressed in NS0 cells
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS
Extra Details:: DCBLD2, was identified through its up-regulation in a highly metastatic lung cancer cell line. DCBLD2 has structural similarities to neuropilins, VEGF receptors, and semaphorins, and is thought to play a role in cell motility and metastasis. SEMA4B is a receptor for DCBLD2/ESDN/CLCP1 and this interaction may be involved in regulating cancer cell metastasis. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using P19 mouse embryonal carcinoma cell line Flow Cytometry: 2.5ug labels 10e6 cells using NCI-H460 human large cell lung carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.