E3500-37F
Escherichia coli, O157
Size
£639.00
- SKU:
- E3500-37F
- Purity:
- Purified by Protein A affinity chromatography from ascites or culture medium.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partially purified LPS preparation from E. coli O157
- Clone:
- 10D9
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- Escherichia coli is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria. Applications: Suitable for use in Immunofluorescence and ELISA. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:10-1:50 ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Matched Pair: Capture: E3500-37F Detection: E3500-37B Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer
- Shipping Conditions:
- Blue Ice