E3486-50
ESAM (Endothelial Cell Adhesion Molecule)
Size
£812.00
- SKU:
- E3486-50
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatent.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gin30-Ala247 from human ESAM, expressed in NSO cells.
- Clone:
- 7H234
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Endothelial cell adhesion molecule (ESAM) is a 55kD type I transmembrane glycoprotein of the JAM family of immunoglobulin superfamily molecules. The 390 aa human ESAM contains a 216 aa extracellular domain (ECD) with a V-type and a C2-type Ig domain. The ECD of human and mouse ESAM share 69% aa identity. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes. ESAM mediates endothelial cell homophilic adhesion and supports neutrophil extravasation through tight junctions. Applications: Suitable for use in Direct ELISA, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: IImmunocytochemistry: 8-25ug/ml Immersion fixed HUVEC human umbilical vein endothelial cells Flow Cytometry: 0.25ug/10e6. HUVEC human umbilical vein endothelial cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice