E3455-23F

Erythropoietin Receptor (EPOr, EPO-R)

Size

£869.00

SKU:: E3455-23F
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Pro26-Pro250 from human Erythropoietin R expressed in NS0 cells.
Clone:: 12K89
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells. Applications: Suitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml with lysates of UT-7 human acute myeloid leukemia cell line under reducing conditions Flow Cytometry: 0.25ug/10e6 cells. Validated with TF-1 human erythroleukemic cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.