E3455-15B
Erythropoietin (EPO) (Biotin)
Size
£1,042.00
- SKU:
- E3455-15B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Ala27-Arg192 from mouse Erythropoietin, expressed in CHO cells.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA per 1ug as a carrier protein. Labeled with Biotin. Reconstitute with 250ul of sterile PBS.
- Extra Details:
- Erythropoietin (Epo) is a 34kD glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three Nglycosylation sites, four alpha helices, and N to Cterminal disulfide bond are conserved across species. Glycosylation of Epo is required for biological activities in vivo. Mature mouse Epo shares 95% aa sequence identity with rat Epo and 73%-82% with bovine, canine, equine, feline, human, ovine, and porcine Epo. Epo is primarily produced in the kidney by a population of fibroblastlike cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells. Ligand induced dimerization of Epo R triggers JAK2mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia- induceable transcription factors HIF1alpha and HIF2alpha. Epo additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice