Erythrocytes

Size

£500.00

SKU:: E3453-46A
Purity:: Purified by DEAE chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization.
Format:: Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Extra Details:: Applications: Suitable for use to sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes. Other applications have not been tested. Recommended Dilutions: Haemolytic complement assays: 1:60 (Haemolytic titre) at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement and 2% RBC, not less than 1:500 when tested with bovine erythrocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.