E3452-59

ERK5 (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7)

Size

£620.00

SKU:: E3452-59
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human Erk5.
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See E3452-59C.
Extra Details:: Erk5, also known as BMK1 (big mitogen-activated kinase 1), has a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the amino-terminal kinase domain with Erk1/2. However, the long carboxy-terminal domain makes Erk5 a unique MAP kinase. Erk5 is activated by a wide variety of extracellular signals, including tyrosine receptor kinases, G-protein-coupled receptors as well as osmotic and oxidative stress (1,2). The direct upstream kinase of Erk5 is defined as MEK5 (1). MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3–5). Growing evidence indicates that the Erk5 pathway is involved in proliferation/differentiation regulation, oncogenesis and neuronal development (1,6–8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000; Mr ~115kD Immunoprecipitation: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice