E3452-45F-APC
ERK1 (Extracellular Signal-regulated Kinase 1, ERK-1, Esrk1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, Mapk3, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, MAPK1, Microtubule-associated Protein 2 Kinase, MNK1, p44, p44erk1, p44-ERK1, p44mapk, p44-MAPK, Prkm3) (APC)
Size
£1,049.00
- SKU:
- E3452-45F-APC
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to a section of the C-terminus of rat ERK1
- Clone:
- ERK-6B11
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggest that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including: RSK (4) , c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK (5) . MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors (6,7,8) . Applications: Suitable for use in FLISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice