E3451-35B

ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Size

£971.00

SKU:: E3451-35B
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Extracellular fragment of recombinant human c-erbB-3/HER-3 oncoprotein. MW of Antigen: 185kD. Epitope: Extracellular domain. Cellular Localization: Cytoplasmic and cell membrane
Clone:: 3F162 (H3.90.6; same as Ab90)
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3451-35BX
Extra Details:: c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas, and colon. Heregulin & EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. Does not block the binding of heregulin to c-erbB-31, has no effect on tyrosine kinase activity1, receptor internalization1, or tumorigenic growth of cells overexpressing the cerbB- 32. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Effect on Heregulin (NDF) Binding (Non-inhibitory) and Effect on Tumor Growth (Non-inhibitory). Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 2ug/mg protein lysate. Use Protein A. Well suited for Immunoprecipitation of unoccupied as well as occupied receptor. Optimal dilutions to be determined by the researcher. Positive Control: T47D cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice