E3451-27A

ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)

Size

£927.00

SKU:: E3451-27A
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant protein corresponding to aa23-652 at the extracellular domain of human ErbB2. UniProt Accession: P04626.
Clone:: 11C99
Format:: Supplied as a liquid in PBS, pH 7.4, 5% trehalose.
Extra Details:: HER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface- bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml (ECL) ELISA: 1:10,000-1:150,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice