E3415-05A
EP-CAM
Size
£876.00
- SKU:
- E3415-05A
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HT-29 colon carcinoma cell line
- Clone:
- HEA125
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
- Extra Details:
- This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumor-specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen and paraffin): Neat. Note: Requires protein digestion pre-treatment of paraffin sections e.g. 0.1% pronase, 10 minutes Western Blot: 1:250-1:2000 Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice