E3412

Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG)

Size

£351.00

SKU:: E3412
Purity:: Purified. ≥ 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS
Extra Details:: Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human. Source: EGF1-52 Biological Activity: The mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.