E3405-50

Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326, CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A, CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Glycoprotein, EGP, EGP40, GA733-2, hEGP-2, KS-1/4 Antigen, KSA, M1S2, M4S1, Major Gastrointestinal Tumor Associated Protein GA733-2, MIC18, MK-1, Tumor-associated Calcium Signal Transducer 1, TACD1, TACSTD1, TROP1)

Size

£691.00

SKU:: E3405-50
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: H69 cell line (small cell lung carcinoma).
Clone:: 10B1416 (VU-1D9)
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: The epithelial cell adhesion molecule (Ep-CAM) is a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen and paraffin):1:200 for paraffin sections. Requires protein digestion pretreatment (e.g. trypsin, pronase) of paraffin sections. Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Western Blot: Detects a band at ~38kD in HT-29 cell lysates. Optimal dilutions to be determined by the researcher. Positive Control (Immunohistochemistry): Large bowel Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice