E3385-BIOTIN
Epiligrin (Laminin 5) (Biotin)
Size
£1,081.00
- SKU:
- E3385-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from cultured supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human keratinocytes
- Clone:
- 2Q588 (P3E4)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Epiligrin, the major component of human keratinocyte extracellular matrix, serves as the preferred integrin ligand for alpha 3 beta 1 in plasma membranes and focal adhesions, and colocalizes with alpha 6 beta 4 in hemidesmosomes. The laminin-5 isoform is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands. Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule. Laminin-5 is synthesized initially as a 460kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix. The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160kD and from 155 to 105kD, respectively. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Paraffin sections Western Blot: Non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice