E3375-47D

Epidermal Growth Factor Receptor, T790M, Recombinant, Human, GST-Tag (erbB, EGFR)

Size

£912.00

SKU:: E3375-47D
Molecular Weight:: 88
Purity:: ~40%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.
Extra Details:: Human EGFR T790M (GenBank Accession No. NM_005228), (aa668-1210) with N-terminal GST tag. Applications: Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Specific Activity: ~0.9 pmol/min/ug Recommended Dilution: Assay Conditions: 50mM HEPES, pH 7.5, 10mM of MgCl2, 1mM of EDTA, 0.01% Brij-35, 200uM ATP. Substrate 2uM Tyr peptide 4 at RT for 1 hour. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.