E3375-35H

Epidermal Growth Factor Receptor (erbB, EGFR, EC 2.7.10.1, ERBB, ERBB1, MENA)

Size

£320.00

SKU:: E3375-35H
Purity:: Purified by Protein G affinity chromatography from cell culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human EGF-Receptor
Clone:: EGF-R2
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.09% sodium azide. Reconstitute with10ul/100ul sterile ddH2O.
Extra Details:: EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a (TGFa), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Paraffin and frozen Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.