E3375-30
Epidermal Growth Factor Receptor (EGFr)
Size
£819.00
- SKU:
- E3375-30
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Epidermal Growth Factor Receptor derived from A431 cells.
- Clone:
- 3G143 (31G7)
- Format:
- Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide.
- Extra Details:
- The epidermal growth factor receptor (EGFr) is a 170kD transmembrane protein consisting of an extracellular EGF-binding domain, a short transmembrane region, and an intracellular domain with ligand-activated tyrosine kinase activity. Two ligands can activate EGFr: epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-a). When TGF-a binds to EGFr, receptor tyrosine kinase activity is upregulated, producing phosphorylation and an increase in cytosolic calcium within target cells. This triggers increased synthesis of DNA and the proliferation and differentiation of target cells. TGF-a is an important effector of malignant transformation in a variety of cells. EGFr is expressed in many normal epithelial tissues, particularly in the basal layers of stratified epithelium and in squamous epithelium. Over-expression of EGFr has been observed in many types of neoplasia, as the result of gene amplification and/or increased protein transcription. EGFr over-expression has been detected in endometrial carcinoma, correlated with myometrial invasion, in lung neoplasms including metaplastic squamous epithelium, squamous cell carcinoma, adenocarcinoma, and neuroendocrine lung tumors, and in glioblastoma multiforme brain tumors. EGFr expression has also been reported in most head and neck carcinomas. Variable EGFr expression has been positively correlated with increasing tumor stage in transitional bladder, renal cell, and gastric carcinomas, and in inverse correlation to estrogen receptor (ER)-negative tumor status in breast carcinoma. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/10e6 cells in 100ul Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections): 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice