E3375-25A

Epidermal Growth Factor Receptor (erbB, EGFR, EC 2.7.10.1, ERBB, ERBB1, MENA) (Not for Export EU)

Size

£307.00

SKU:: E3375-25A
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human native EGF-Receptor
Format:: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Extra Details:: The EGF receptor (EGFR), a 180kD transmembrane tyrosine kinase, is part of the ErbB family which mediate ligand induced signaling as heterodimers. Ligand binding results in activation of the intrinsic kinase activity and autophosphorylation on tyrosine residues within and surrounding the kinase domain. The phospho-tyrosine residues serve as docking sites for adapter proteins that nucleate signaling complexes that result in the activation of the Ras, PI-3 kinase and PLC-g pathways. EGFR, which transduces the biological effects of EGF and TGF-a, is frequently over-expressed in solid tumors. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunofluorescence: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.