E3375-14-ML650
Epidermal Growth Factor Receptor (erbB, EGFR), Neutralizing (MaxLight 650)
Size
£1,023.00
- SKU:
- E3375-14-ML650
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human A431 membrane proteins.
- Clone:
- 0.T.37 (LA1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Neutralization. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Acetone-fixed tissues. Neutralization of Biological Activity: 10ug/ml causes a 74% reduction in cell number in a 5 day cell proliferation assay using A431 cells. Western Blot: 0.5-2ug/ml detects non-reduced EGF receptor in RIPA lysates from non-stimulated human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3375-14 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemilum-inescence detection system. Immunoprecipitation: 4ug immunoprecipitates the EGF receptor from 500ug of non-stimulated A431 RIPA lysate. Immunocytochemistry: 10ug/ml shows positive immunostaining for the EGF receptor in A431 cells fixed with 4% para-formaldehyde. Optimal dilutions to be determined by the researcher. Biological Activity: Inhibits A431 cell growth. Recognizes an antigenic determinant located on the extracellular domain of the receptor. Positive Antigen Control: A0001-50: A431 Cells, Non-Stimulated Use 20ug per lane for minigels. Storage and Stability: May be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice