E3375-10F-ML650
Epidermal Growth Factor Receptor (erbB, EGFR) (MaxLight 650)
Size
£960.00
- SKU:
- E3375-10F-ML650
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Extracellular domain of human EGF-receptor from head and neck carcinoma.
- Clone:
- 3H2090 (ICR10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2,-3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Recommended Secondary Reagent: I1904-49H: IgG, F(ab')2, H&L X-Adsorbed (HRP) RbxRt I1904-47H: IgG, H&L (HRP) Pab GtxRt Positive Control: Breast carcinoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice