E3375-10F-HRP
Epidermal Growth Factor Receptor (erbB, EGFR) (HRP)
Size
£960.00
- SKU:
- E3375-10F-HRP
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Extracellular domain of human EGF-receptor from head and neck carcinoma.
- Clone:
- 3H2090 (ICR10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2,-3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Immunoprecipitation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Recommended Secondary Reagent: I1904-49H: IgG, F(ab')2, H&L X-Adsorbed (HRP) RbxRt I1904-47H: IgG, H&L (HRP) Pab GtxRt Positive Control: Breast carcinoma Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice