E3375-07X-ML650

Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain (BSA & Azide Free) (MaxLight 650)

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£1,119.00

SKU:: E3375-07X-ML650
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HC2 20 d2 cells. Cellular Localization: Cell membrane.
Clone:: 0.N.269
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000 EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a (TGF-a), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is over expressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in FLISA, Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml for 2 hrs at RT. Suitable for Western blot of extracellular domain of EGFR. Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein G. Immunohistochemistry (Formalin/ Paraffin): 2-4ug/ml for 30 mniutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 min at 37°C. (Note: E3375-06B/ E3375-06BX are more suitable for use in IHC). Optimal dilutions to be determined by the researcher. Positive Control: A431 cells, placenta or squamous cell carcinoma. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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