E3375-07X-BIOTIN

Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain (BSA & Azide Free) (Biotin)

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£1,119.00

SKU:: E3375-07X-BIOTIN
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HC2 20 d2 cells. Cellular Localization: Cell membrane.
Clone:: 0.N.269
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a (TGF-a), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is over expressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml for 2 hrs at RT. Suitable for Western blot of extracellular domain of EGFR. Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein G. Immunohistochemistry (Formalin/ Paraffin): 2-4ug/ml for 30 mniutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 min at 37°C. (Note: E3375-06B/ E3375-06BX are more suitable for use in IHC). Optimal dilutions to be determined by the researcher. Positive Control: A431 cells, placenta or squamous cell carcinoma. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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