E3375-06C
Epidermal Growth Factor Receptor (erbB, EGFR)
Size
£934.00
- SKU:
- E3375-06C
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Extracellular domain of human recombinant EGFR protein. Cellular Localization: Cell membrane.
- Clone:
- 199.12
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3375-06CX.
- Extra Details:
- EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1,-2,-3-4 or HER-1,-2,-3-4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein A. Western Blot: -4ug/ml for 2hrs at RT Optimal dilutions to be determined by the researcher. Positive Control: A431 cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice