E3375-01P-BIOTIN

Epidermal Growth Factor Receptor (erbB, EGFR) (Biotin)

Size

£876.00

SKU:: E3375-01P-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human epidermoid carcinoma line A431. Epitope: Between amino acid residues 6-273 of human EGFR. Cellular Localization: Cell Membrane and Secreted.
Clone:: EGFR1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide. Labeled with Biotin.
Extra Details:: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:2000 Flow Cytometry: 1:10-1:1000 Immunofluorescence (IC): 1:10-1:500 Immunohistochemistry: 1:10-1:500 Optimal dilutions to be determined by the researcher. Positive Control: EGFR Knockout HeLa Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice