E3371-01B
Ephrin B, pan
Size
£767.00
- SKU:
- E3371-01B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the ephrin-B sequence. Species sequence homology: mouse and rat
- Clone:
- 6A72 (2D3E9)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Ephrins are a family of proteins that bind to the tyrosine kinase Eph receptor. Their function is still under investigation, but they are suspected to play an important role in morphogenesis. The ephrins can be grouped into two classes: those that are tethered to the membrane by a GPI linkage, called ephrin-A proteins (which bind to EphA receptors), and those with a membrane-spanning region, called ephrin-B proteins (which bind to EphB receptors). Ephrins are expressed in the embryo in various tissues in the forming mesoderm, endoderm and nervous system in all of the vertebrate species in which they have been identified. Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 is a member of the ephrin-B protein family. Ephrin-B3 binds EphA4, EphB1, EphB2 and EphB33,. Ephrin-B1 is one of the transmembrane ligands for the EphB2 receptor. In the embryo, EphB2 and ephrin-B1 participate in neuronal axon guidance, neural crest cell migration, the formation of blood vessels and the development of facial structures and the inner ear. EphB2 and ephrin-B1 can both signal through their cytoplasmic domains and become phosphorylated when bound to each other. Tyrosine phosphorylation regulates EphB2 signaling and likely also ephrin-B1 signaling. Applications: Suitable for use in Western Blot, Immunoprecipitation, ELISA and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry (ICC/IF): 2ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: Human 293T and MCF10A lysates, chicken cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice