EP3 Receptor

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£754.00

SKU:: E3364-32
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 15aa from the N-terminal, extracellular domain of the rat EP3 Receptor (KLH).
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3 and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR-7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus. EP3 is expressed in the kidney, brain, stomach, uterus, liver, skeletal muscle, intestine and thymus. Alternative splicing of EP3 produces various isoforms with different C-terminal tails. EP3 isoforms have identical ligand binding but different coupling properties with G-proteins: alpha and beta coupled to Gi, whereas gamma couples with Gi and Gs. EP3 knockout mice show impaired febrile response to pyrogens. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml using 1ug/ml of control peptide E3364-30 Western Blot: 1-10ug/ml using ECL. EP3 is ~62kD protein in the kidney. Immunohistochemistry: 2-20ug/ml. Reported to work with mouse and human retina and human gastric tissues (7, 8). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice