EP2 Receptor

Size

£754.00

SKU:: E3364-27
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated 14-amino acid peptide within the 1st extracellular, N-terminal domain of human EP2 receptor. Species sequence homology: rabbit: 85%; canine: 71%. This sequence is not conserved in other species. No significant sequence homology with EP1, EP3 or other GPCR.
Format:: Supplied as a powder lyophilized from a solution containing PBS, 0.1% BSA and 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Extra Details:: Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2 and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin reledid not ase, and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR-7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. Mr ~67kD. ELISA: 0.5-1ug/ml using control peptide (E3364-26) to coat plates at 1ug/ml Optimal dilutions to be determined by the researcher. Control Peptide: E3364-26: EP2 Receptor, Human, Control Peptide Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice