E3362-ML650
Ephrin A4 (EFNA4) (MaxLight 650)
Size
£991.00
- SKU:
- E3362-ML650
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the C-terminal end of the mouse ephrin-A4 protein.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells, formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors. Ephrin-A4, also known as LERK-4 and EFL-4, is a Class A protein. It has been shown to bind the EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 receptors. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/mL Immunoprecipitation: 5-10ug/ IP reaction Immunohistochemistry (FFPE): 4-10ug/ml. Heat induced epitope retrieval with EDTA, pH 8.0 was required for optimal staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice