E3360-02B-ML490
EphA2 Receptor (Ephrin A2 Receptor, EphA2, EPHA2, EPH Receptor A2) (MaxLight 490)
Size
£1,042.00
- SKU:
- E3360-02B-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the C-terminal region of the human EphA2 Receptor protein.
- Clone:
- 6A69
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The Eph receptors are the largest family of tyrosine kinases. They can be grouped into two subclasses based upon sequence homology: the EphA and EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B lignads, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic development, including in the nervous and vascular systems. Ephrins and Eph receptors have been implicated in many developmental processes, including neuronal network formation, guidance of cell migration and axonal pathfinding. They remain expressed at lower levels in adult tissues, and are upregulated in pathological conditions such as cancer. EphA2 has been previously named Eck, Myk2 and Sek2. It binds to ephrin-A1, -A3, -A4 and –A5, and is expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung and ovary. Applications: Suitable for use in FLISA, Immunoprecipitation, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Positive Controls: Human EphA2-transfected 293 and MDA-MB-231 cells. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice