E3360-01D
Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2) [KO Validated]
Size
£889.00
- SKU:
- E3360-01D
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln25-Asn534 from human Eck expressed in NS0 cells.
- Clone:
- 7H150
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Eck (EphA2) is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1-A5. A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976aa precursor including a 24aa signal sequence, a 510aa extra- cellular domain (ECD), a 24aa transmembrane segment and a 418aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM). The ECD of human EphA2 shares 90-94% aa sequence identity with mouse, bovine, and canine EphA2 and ~45% aa sequence identity with human EphA1, 3, 4, 5, 7 and 8. EphA2 becomes autophosphorylated following ligand binding and then interacts with SH2 domain- containing PI3-kinase to activate MAPK pathways. Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin and can be induced by p53 family transcription factors. EphA2 is upregulated in breast, prostate and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, and direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug labels 10e6 A431 human epithelial carcinoma cell line. Immunocytochemistry: 8-25ug/ml detects Eck on immersion fixed A431 human epithelial carcinoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice