E3351-07E
Eotaxin-2 (CCL24)
Size
£512.00
- SKU:
- E3351-07E
- Purity:
- Purified by Protein A affinity chromatographyd from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Eotaxin-2, expressed in E. coli.
- Clone:
- 5C38
- Format:
- Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Eotaxin-2 induces chemotaxis of eosinophils and basophils, and the release of histamine and leukotriene C-4 from basophils primed with IL3. Eotaxin-2 activates chemotaxis-related events such as actin polymerization, and the respiratory burst almost as efficiently as Eotaxin. Monoclonal antibodies (7B11) selectively blocking the eotaxin receptor, CCR3, also block migration and release responses. Intradermal injection of eotaxin-2 in a rhesus monkey (100 or 1,000 pmol per site) induces a marked local infiltration of eosinophils. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 2-4ug/ml Western Blot: 0.25-0.5ugml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice