E3330
Eosinophil Major Basic Protein
Size
£685.00
- SKU:
- E3330
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human eosinophil major basic protein (MBP).
- Clone:
- 1.B.787 (BMK13)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
- Extra Details:
- Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Reacts with fresh frozen tissues that were sectioned (6um), dried for 1 hour at RT and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. Immunohistochemistry (FFPE): 4% PFA (3hr) should be used for skin biopsies. 5uM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues. Western Blot: Purified normal human eosinophils were treated with digitonin (5mg/ml) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~14kD, and typical human samples range from 10-16kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice