EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Size

£890.00

SKU:: E3321
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Linear peptide corresponding to mouse EOMES, conjugated to KLH. Epitope: C-Terminus
Format:: Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.05ug/ml detected EOMES in 10ug of E13-14 mouse brain lysates. Immunohistochemistry (Paraffin): 1:100 from a previous lot detected EOMES in mouse and human brain. Requires HIER with citrate buffer, pH 6.0 Immunohistochemistry (Paraffin): 1:1000 from a representative lot detected EOMES in mouse cerebral cortex and mouse cerebellum tissue. Requires HIER with citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher. Positive Control: E13-14 mouse brain lysate Recommended Secondary Antibodies: I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.